This is a project which is currently making use of HPC facilities at Newcastle University. It is active.
For further information about this project, please contact:
This project is designed to beta test the 10X genomics scRNA-seq pipeline on comet.
The following analysis steps will be undertaken.
* Conversion of raw data (illumina bcl files) to sample FASTQ with bcl2fastq.
* Quality control of FASTQ files with FastQC and Fastq Screen.
* Generation of full output files for all samples from FASTQ files with 10X CellRanger software.
* QC of the outputs from the 10X software.